Among the multiple experimental animal models employed for analyzing the various aspects of mammary carcinogenesis, the induction of mammary tumors in rats experimentallyinduced mammary tumors in rodents have proven to constitute useful tools for the study of the pathogenesis of cancer and of the molecular mechanisms involved in the initiation and progression of the neoplastic process. Tumor induction in research animals is a critically important experimental activity which requires consideration of the effect of the tumors on the animal. I was using animals from charles rivers labs but recently janvier labs has came with. Using the standard protocol for induction of gastric cancer, rats were given carcinogen mnng at dose 200 mg kg body weight once daily by oral gavage for 2 weeks, followed by oral administration of nacl 1 mlrat once every 3 days for 4 weeks. Here we describe studies using 7,12 dimethylbenzaanthracene dmba to investigate the role of obesity in dmbainduced mammary tumor susceptibility in the female zucker rat fafa, which is the most widely used rat model of genetic obesity. Histogenesis and growth pattern of 1,2dimethylhydrazineinduced rat colon.
Use of a chemically inducedcolon carcinogenesisprone apc. Induction of experimental mammary carcinogenesis in rats with 7,12. This is a pdf file of an unedited manuscript that has been. Links to pubmed are also available for selected references. Chemical induction of ovarian tumors in rats 55 it has been estimated that up to 90% of all neoplasms may have antecedent environmental determinants, e.
Obesity promotes 7,12dimethylbenz a anthraceneinduced. These bioassays involve the use of colon carcinogenesis models which mainly consist of animal xenografts, an adenomatous polyposis coli apcmutant mouse model and a chemicallyinduced crc model. Induction of ovarian cancer in rats was carried out using 7,12dimethylbenz a anthracene dmba coated on silk suture and then implanted in the left ovary of female wistar rats. High body mass index has been associated with increased risk for various cancers, including breast cancer. Induction of intestinal tumours in rats by chrysazin. Mammalian models of chemically induced quantitative imaging in. Further, in den2aaftreated rats, the antiproliferative activity of gse was also associated with the induction of apoptosis as evidenced by increasing the number of tunel and m30 cytodeath. Chemical induction of ovarian tumors in rats sciencedirect. Mammary carcinogenesis was induced in 36 female holtzman rats by providing a single subcutaneous injection.
August 2016 general tumor induction in research animals is a critically important experimental activity which requires consideration of the effect of the tumors on the animal. Studies on the induction of bone and soft tissue tumours in rats by gamma irradiation and the effect of growth hormone and thyroxine. The carcinogenicity of multiple intragastric doses of aromatic and heterocyclic nitro or amino derivatives in young female spraguedawley rats. The development of effective chemopreventive agents against cigarette smokeinduced lung cancer could be greatly facilitated by suitable laboratory animal models, such as animals treated with the tobaccospecific lung carcinogen 4methylnitrosaminopyridyl1butanone nnk.
Tumor induction in mice and rats iacuc guideline effective date. Chemoprevention of dmba induced mammary tumors in rats by a. Hi, i am working with cancer xenograft models in nude mice. This rat model develops a high incidence 85% of rats induced and. The use of animal models for cancer chemoprevention drug. Figure 2 magnetic resonance imaging mri monitoring of dynamic tumor growth patterns of primary liver cancer model in sprague dawley rat sd rats generated. In the current study, we established a novel lung cancer model in wistar rats treated with nnk. Articles esophageal cancer prevention in zincdeficient rats. The possible protective effect of piper aduncum capsule on dmba dimethylbenz. Mammalian models of chemically induced primary malignancies. Induction of colon tumors in rats through aromatic amines such as 3. Chemopreventive activity of mgn3biobran against chemical. Pdf induction of mammary gland tumor in female spraguedawley. I was using animals from charles rivers labs but recently janvier labs has came with the cheaper offer for their strain of balbc nude mice.
Under conducive conditions, which were readily reproduced, multiple mammary carcinomas and these tumors only were induced in every rat in repeated experiments in 60 days or less. Development of hemangiosarcomas in b6c3f1 mice fed 2methyl1nitroanthraquinone. In these studies, drinking water administration of 4nqo 20 ppm for 10 weeks to f344 rats induced a range of premalignant and invasive malignant lesions hyperplasia, squamous. Experimentally induced mammary tumors in rats springerlink.
Pdf induction of experimental mammary carcinogenesis in rats. Induction of tumors in rats with procarbazine hydrochloride jnci. A study was made of the optimal conditions for the induction of mammary cancer in the rat. Pmc free article morichavez p, upton ac, salazar m, conklin jw. Induction of tumors in rats with procarbazine hydrochloride. Protective effect of piper aduncum capsule on dmbainduced.
Two separate oral cancer induction experiments table 1 were performed to generate tissues for molecular analysis. This la7 cellinduced rat mammary gland tumor model may be useful for studies in breast cancer drug or nutraceutical research and development. It was proposed that chemical substances applied to the vulvovaginal areas for a variety of purposes could ascend through the vagina, cervix, uterus, and fallopian tubes to reach the ovarian surface 4. Concomitantly with chemical induction, the rats were given biobran alone group 2 at a dose.